Bright-Dtech™ 980 – Yb (Infrared) Coupled to Anti-Rabbit IgG (H + L)
Description
With Bright-Dtech™, we offer you an innovative solution to have a higher detection sensitivity with easy-to-use protocols, applicable in various molecular and cellular biology techniques. They are ultra-efficient fluorescent nano-molecules with unique characteristics.
Secondary antibodies aim to detect and visualize a primary antibody that is bound to its protein of interest. Rabbit antibody is often used in applications such as ELISA, Western Blot, IHC, or cell imaging.
If you are using a primary antibody produced in rabbits, you will choose as the secondary antibody an anti-rabbit antibody produced in a species other than rabbit.
Due to lanthanide’s fingerprinting emission spectrum, Bright-Dtech™ – Yb (Infrared) can be incorporated with other Bright-Dtech™ nanoparticles to achieve multiple detection of biomarkers or proteins in the same biological sample.
Requested Equipment
Additional Documents
Publications and references
Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117
Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007
Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069
Additional information
| Weight | 0.7 kg |
|---|---|
| Dimensions | 14 × 15 × 11 cm |
| Volume | 250 µl, 500 µl, 1 ml, 1,5 ml, 2 x 1,5 ml, 3 x 1,5 ml, 4 x 1,5 ml |
| Concentration | 10 nM |
| Color | Infrared |
| Coupled to | Anti-Rabbit IgG (H + L) |
| Nanoparticle | Ytterbium (Yb) |
| Product | Bright-Dtech |
| Wavelenght | 340ex. – 980em. |
