With Link-Dtech™, we offer you an innovative solution to have a higher detection sensitivity with easy-to-use protocols, applicable in various molecular and cellular biology techniques. They are ultra-efficient fluorescent nano-molecules with unique characteristics.
HK-Dtech ensures simple and performant coupling. Kits are designed to be unique coupling with an analyte, an antibody and other biologicals. In addition, you can custom as you want the surface of the nanoparticles. If you need to be a specific coupling, please to contact us : firstname.lastname@example.org
Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière Chempluschem. 2016 Jun;81(6):497.
Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces. Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt Chemistry. 2020 Nov 17;26(64):14602-14611.
Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h. Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian Bioconjugate Chem. 2018, 29, 4, 1327–1334.